![]() ![]() ![]() This unique sand-mediated cryopreservation method may greatly facilitate the convenient and ready availability of high-quality hiPSCs and probably many other types of cells/tissues for the emerging cell-based translational medicine. during the last glacial cycle: form, flow, streams and lobes. explained by substrate condition, channel form/flow type and floodplain connectivity. Furthermore, the cryopreserved hiPSCs retain high pluripotency and functions judged by their pluripotency marker expression, cell cycle analysis, and capability of differentiation into the three germ layers. Management implications and relevance of this study. Here, sand from nature is discovered to be capable of seeding ice above −10 ☌, which enables cryopreservation of hiPSCs with no serum, much-reduced cryoprotectant, and high cell survival. ![]() The latter may cause spontaneous differentiation and/or introduce xenogeneic factors, which may compromise the quality of hiPSCs. However, contemporary methods for hiPSC cryopreservation are associated with both limited cell survival and high concentration of toxic cryoprotectants and/or serum. Human induced pluripotent stem cells (hiPSCs) possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use. ![]()
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